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We report the novel specific marker for lymphatic endothelium, lymphatic vessel endothelial hyaluronan receptor (LYVE-1), in prostatic, benign prostatic hyperplasia (BPH) and prostate cancer (PCa) tissue.
Podoplanin, a representative immunohistochemical marker for lymphatic endothelial cells, is also expressed in many other kinds of cancer cells, although its pathophysiological function is largely unknown.
Immunostaining with D2-40 monoclonantibodyody which is a specific marker for lymphatic vessel elucidated tumor invasion to lymphatic vessels (C).
In order to corroborate the role of prox1b in lymphangiogenesis, the expression of lyve1, a specific molecular marker for lymphatic endothelial cells [14], [16], has been assayed (Figure 4).
Therefore, SHARP1 may be a novel marker for lymphatic metastasis in EC patients.
Besides podoplanin, LYVE-1 is a well-established marker for lymphatic vessels [ 5].
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Among them, LYVE-1 is a reliable specific marker for lymphatics [ 4, 5].
Novel selective markers for lymphatic endothelium have been released, such as LYVE-1, Prox-1, desmoplakin, and podoplanin [ 7- 12].
Immunohistochemical (IHC), Western blot, and immunoelectron microscopic studies were conducted using D2-40 as markers for lymphatic vessels, as well as CD34 for capillary blood vessels.
Currently, the available endothelial markers for lymphatic vessels include vascular endothelial growth factor receptor-3 (VEGFR-3), LYVE-1, Podoplanin, Prox1, and others.
Recently, several markers for lymphatic endothelium have been discovered, including transmembrane protein lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), and these provide powerful tools for studying tumor lymphangiogenesis.
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