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These features make VP88GFP an excellent marker for generating GFP-tagged recombinant SGIV for the experimental analysis and real-time visualization of SGIV infection.
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In this study, the applicability of the SBP marker technology for generating markers is shown for improving a genetic map that represents polymorphisms between two Arabidopsis ecotypes, Col-0 and Nd-0.
Post-translational modifications of histone tails are important regulatory markers for generating transcriptionally active and inactive chromatin.
Here a sequence based polymorphic (SBP) marker technology for generating molecular markers for targeted genomic regions in Arabidopsis is described.
The genetic approaches taken include selection (for heat tolerance and resistance to nematicides); screening for natural isolates displaying heat tolerance; hybridization (aided by genetic markers) and mutagenesis for generating mutants displaying either desired beneficial traits or marker mutations.
Small RNA fraction was extracted from the gel slice corresponding to the delimiting markers and used for generating libraries for deep sequencing.
Also, data presented here provide an important tool for generating markers to saturate melon genetic maps.
Whereas this allows for addressing research questions at the level of entire genomes [ 13, 32, 33], the methods used also provide resources that can be used for generating markers for more specific purposes.
CRISPRm should serve as a rapid and high throughput means for connecting the genotypes of these organisms to their phenotypes, that is for generating marker-free barcoded alleles for large-scale pooled fitness studies of loss-of-function mutants in these organisms (Giaever et al., 2002).
One is pJS-M14 based on the pMF1 vector system for generating marker-free transgenic plants [ 22], in which expression of the reporter genes GFP and GUS was driven by the apple Rubisco-promotor [ 23]; resistance to the selectable agent kanamycin is provided by a gene fusion between NPTII [ 24] and CodA driven by the 35S CaMV promoter.
Novel methods for generating a marker field in turbulent premixed combustion are described.
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