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Higher sequence variation of the Alu elements was described previously as a marker for gene duplication events.
Due to smaller standard deviations between the 2-ΔΔCt over 3 independent experiments, the beta-actin gene was used as the reference marker for gene expression normalization.
Although we used the Bt protein Cry1Ac as a marker for gene flow from Bt cotton, we note that some cotton grown in Arizona produces two Bt proteins: Cry1Ac and Cry2Ab.
Again, Cerulean was chosen as it acts as an additional marker for gene insertion as well as providing a simple assay for detecting the presence of any frame shifts after the rescuing last beta strand as any frame shifts would abrogate Cerulean fluorescence.
Although it is not strictly necessary for the inserted gene to be fluorescent as gene insertion into our plasmid expression vector will induce Venus fluorescence for screening, hcRed was chosen because it also expresses fluorescence and can be used as a simple additional marker for gene insertion.
A further advancement in this field was the use of IHC as a surrogate marker for gene expression profiling.
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In as far as regulation by microRNAs serves as a marker for genes partially under homeostatic control, we would expect microRNA-regulated genes to enjoy some duplicability advantage.
Histone H3K9 acetylation and H3K4 methylation are markers for gene activation.
We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disruption experiments and the ability to efficiently rescue the marker leaving an unmarked mutation on the chromosome.
H3K9me2, H3K9me3 and H3K27me3 are well-known epigenetic markers for gene repression.
However, B. cenocepacia strain J2315 is difficult to manipulate genetically, in part due to its high level of antibiotic resistance, which precludes the use of the most common selectable markers for gene exchange.
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