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To assess the rate of spontaneous differentiation in these cells, expression of the cornified envelope precursor involucrin, a commonly used marker for differentiation, was analysed.
Closer inspection of many antibody-stained colonies revealed that there were rare transition regions that simultaneously expressed OCT4, the gold-standard marker for pluripotency, and full-length MUC1, which appears to be a marker for differentiation.
These findings classify MS4A8A/MS4A8B as a novel marker for differentiation in the gut.
Increase in cytokeratin 8/18 expression was used as the marker for differentiation (Peehl et al, 1993; Hsieh et al, 1995).
The number of telogen hairs, as an additional marker for differentiation between androgenetic alopecia from the female pattern type and diffuse hair loss, allows a more exact classification.
Kehlen et al (2003) have shown that CD13 expression in undifferentiated thyroid carcinomas was higher than that in papillary or follicular thyroid carcinomas, suggesting that it is a marker for differentiation.
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Additionally, the ability to evaluate differentiation has been challenged by a lack of markers for differentiation.
Next, we examined markers for differentiation after 1, 3 and 5 days of BMP treatment.
There is a growing interest in the use of anti-glycan antibodies as markers for differentiation and stratification of CD.
Lymphoid expression of several markers for differentiation status were examined (supplementary Table S5), for line 131 no differences from controls were observed for any of the lymphoid tissues and antibodies tested (data not shown), suggesting that for this line the status of the B and T cells (with respect to the antibodies used) is not modified by the presence of the transgene.
Genetic markers for differentiation at these scales included partial sequences of mitochondrial cytochrome- c-oxidase subunit I, and an array of nuclear DNA microsatellite loci [ 39].
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