Previous studies have shown a complex interaction between different immune markers, highlighting the need for combined marker analysis (de Kruijf et al, 2010a, 2010b; Zeestraten et al, 2011).
In this study, quantification of OPC in the stab-injured cortex was confirmed by using PDGFRα positivities as the most specific marker for OPCs combined with co-expression of NG2 and FABP7.
In support to the present results, we previously observed negative correlations between XARcomp and the POP proxy marker p,p'-DDE for combined European study groups [ 36].
This new combined marker is labeled COCOC that stands for combined coccolith proxies.
The true positive rate increased with stage for each marker and for the combined two-marker blood test (either methylation marker positive).
For this reason, we established two additional maps containing the codominant markers, as anchor markers, combined with either male or female markers in coupling phase.
Here, methods also exist for combining markers in non-linear or non-parametric fashion [ 35].
In another study evaluating the potential value of the methylation markers CADM1 and MAL as a triage tool for hrHPV+ women, it was found that there is a solid reasoning for combining markers which relate to different stages in cervical carcinogenesis [ 69].
For microsatellite genotyping, two to 10 markers were combined for multiplex PCR amplification based on size and amplification conditions and analyzed on an automated sequencer (ABI 3700, PE Applied Biosystems, Foster City, CA).
Furthermore, the categorisation of prolonged delay (i.e., waiting >3 months before presentation) was identified in the original study as a marker for all cancers combined (Forbes et al, 2014).
