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Similarly, choline acetyltransferase (ChAT), a constitutive marker for cholinergic nerve terminals, was affected only by combined exposure to both terbutaline and CPF.
After that, the cells of each well were incubated separately with the primary antibodies of class III beta tubulin (marker for post-mitotic neurons), and choline acetyltransferase / ChAT (marker for cholinergic neurons) both diluted in blocking buffer at dilution (1 200; Abcam) and incubated overnight at 4 °C.
Choline acetyltransferase (ChAT) is the enzyme responsible for acetylcholine synthesis and ChAT activity has been shown to be a reliable marker for cholinergic integrity [ 8].
Choline acetyltransferase, a constitutive marker for cholinergic nerve terminals, showed only minor CPF-induced changes during the period of rapid synaptogenesis.
In addition, these cells stained negative for serotonin (a marker for serotogenic neurons) and ChAT (a marker for cholinergic neurons), consistent with the reprogrammed cells being DA neuron-like cells.
To this end we used the pan-neuronal marker F25B3.3::GFP (19) (strain LM111) and the marker for cholinergic neurons unc-17::GFP (strain LM112) to facilitate neuronal identification.
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Choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (vAChT) are essential for cholinergic neurotransmission and antisera to these proteins are phenotypic markers for cholinergic neurons [54], [73].
Beyond the validation of [18F]FEOBV PET as a marker for quantifying cholinergic survival, the concordance of the two measures further supports the notion that ChAT and VAChT are regionally co-expressed and highly correlated, both under normal and pathological conditions [32].
(A ) Western blots of microdissected striatum from 10 week old control and Dlx-CKO mice for markers of cholinergic, GABAergic, and dopaminergic signaling.
Acetylcholinesterase activity, a marker of cholinergic dysfunction, was increased by 1.8 fold in the cerebral cortex of diabetic rats.
We investigated the relation of SAI, a putative neurophysiological marker of cholinergic activity, to memory and executive functions in aging.
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