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With targeted insertion near transcriptional termination site, puromycin expression is induced to serve as selection marker for cells containing established targeted insertion.
On immunostaining, fibromuscular cells showed the typical staining pattern with antibodies against smooth muscle myosin (Fig. 2b), smooth muscle actin (Fig. 2c), and Vimentin, a marker for cells of mesenchymal origin (Fig. 2d).
Cell purity was verified by histochemical staining for nonspecific esterase, a marker for cells of the monocytic/macrophage lineage.
The Golgi was examined in cells that expressed the viral capsid protein VP1, which is made late in replication and serves as a marker for cells replicating NV.
The continuous serial sections used in the cell proliferation assay were also immunostained for cleaved Caspase-3, a known marker for cells committing apoptosis (Figure 9B).
The cellular phenotype of the transfected cells (GFP+) was identified by immunostaining for PCNA (Fig. 7 A), a marker for cells in early G1/S phase, or the T-box brain (Tbr) 2 (Fig. 7 B), a transcription factor and marker for cells actively proliferating in the upper layer of the ventricular zone (VZ) and the sub-ventricular zone (SVZ) [22], [23].
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Release of LDH from cells into the surrounding medium is a typical marker for cell death.
Therefore, supernatant LDH of cultured HAECs is detected as a marker for cell injury [36].
(1) Holdenrieder S, Stieber P, Bodenmuller H, Fertig G, Furst H, Schmeller N, et al. Nucleosomes in serum as a marker for cell death.
Figure 5a and b shows that, compared with fresh air, CS exposure rendered cells less capable of reducing resazurin, a dye commonly used to determine the metabolic activity of cells as a marker for cell viability [23].
Deformation index is defined as ratio of both axis of cross sectional area of a deformed cell has been linked with cell deformability and was proved to be an efficient biophysical marker for cell state.
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