To estimate the degree of disruption of the alveolar basal membrane, we analyzed the distribution of E-cadherin, as a marker of basolateral cell polarity, and of laminin, as marker for basement membrane integrity.
This notion was further supported by observation of transverse views with laminin staining as a marker for basement membrane, which showed that SU5402-treated leader cells were apicobasally polarized (Fig. 6D,E).
In this study, collagen IV and NG2 were used as markers for basement membrane and pericytes, respectively.
This fragment may be a useful marker for degradation of the basement membrane in tissue.
ULN, increased in tumour patients, could be a potential clinical marker for monitoring the turnover of basement membrane in tumours.
The cells were then stained for basement membrane protein laminin-5 (10 μg/ml, mouse, human specific, AbCam, Cambridge, MA, USA), apical marker GM130 (10 μg/ml, rabbit, AbCam, Cambridge, MA, USA) and nuclei (Hoechst 33342).
Opening for Basement Jaxx.
Search for "basement watchdog".
In addition, staining for laminin, a marker of the basement membrane, revealed that the vascular architecture was mostly intact in ACN/neo tumours, while aspects of focal basement membrane destruction and alterations in the microvascular architecture were frequent in the inner portion of ACN/IFN-γ tumour.
Samples were immunostained for JAM-C, E-cadherin (a marker for adherens junctions) and laminin α2 (a component of SC basement membrane).
Urinary transferrin, which results from abnormal permeability of the glomerular basement membrane, is suggested to be a marker for early stages of glomerular diseases.
