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Similarly, cells from chick and mouse embryos at this stage are unable to maintain pluripotent marker expression when ERK signaling is inhibited.
This method can be used to determine the optimal cutoff points for protein marker expression when scored semiquantitatively, as was done in this study.
Although HAT p300 deletion does not affect self-renewal in undifferentiated mESC, it causes abnormal marker expression when differentiation is induced [ 9].
Cells did not show significantly different proinflammatory (TNF-α and IL-1β) marker expression when seeded on different scaffolds compared to positive control (p < 0.01, n = 6, Figure 2, Table 1).
Similarly, the ratios did not reveal any novel patterns in marker expression when analysed by group or individual for either biopsy site or nominal biopsy time effects, compared to the total- or phospho-marker levels alone (data not shown).
In SCC9, SCC12B.2 and SCC27 cells and oral keratinocytes the relative levels of marker expression when compared to epidermal keratinocytes were largely the same as in unfractionated cells (Fig. 3E).
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MSChUCB1 was found to react best at upregulating marker gene expression when incubated with CIM.
In addition, no correlation between metastatic status and epithelial marker expression was seen when examining lymph-node positive and lymph-node negative tumors [40].
Efficient rescue of neural crest/placodal marker expression was also observed when Slug was activated at stage 11, but rescue was much less efficient when Slug was activated at stage 13 (FIG. 2; Table 2).
Conversely, these results were not significantly different when marker expression was evaluated in tumor cells rather than stroma.
The results of the study demonstrated that in comparison to wild-type DCs, MyD88-deficient DCs had lower surface marker expression and decreased cytokine secretion when they were incubated on a range of biomaterials.
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