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Cell shape features, surface design parameters, and osteogenic marker expression were strongly correlated in vitro.
Combined with sustained drug availability, more significant changes in cell morphology and enhancement of neural marker expression were observed.
The results showed that alkaline phosphatase (ALP) activity, mineralization, and osteogenic marker expression were significantly improved with hPMSCs cultured in the hFDM-coated mesh scaffolds compared to the control and fibronectin-coated ones.
So to better examine cell fate, shifts in MSC lineage marker expression were monitored over time.
The LPS-induced cytokine production and cell surface marker expression were characterized by flow cytometric analysis.
PCR reactions to assess EMT marker expression were performed on amplified cDNAs using the following primers: GFAP (glial fibrillary acidic protein) forward: 5'-ACAGACTTTCTCCAACCTCCAG- 3'.
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Double-knockdown hESCs (ShD1D2, ShD2D3, and ShD1D3-hESCs) showed a propensity for spontaneous differentiation into cells expressing endoderm markers, whereas pluripotency and neuroectoderm marker expression was systematically diminished.
CD68 marker expression was found in scattered subepicardial cells, and some of them co-expressed the Lyve1 antigen (Fig. 9f j).
Both haematoxylin-eosin analysis and CD45 marker expression are in agreement with these findings.
The viability, neurite outgrowth and neural marker expression was found to be comparable between the conventional poly-l-ornithine (PLO) surface and the CNT surface.
In this study, the surface marker expression was investigated on dental pulp stem cells (DPSCs), dental follicle cells (DFCs), periodontal ligament stem cells (PDLSCs), and buccal mucosa fibroblasts (BMFs) utilising surface markers for flow cytometry.
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