Double-knockdown hESCs (ShD1D2, ShD2D3, and ShD1D3-hESCs) showed a propensity for spontaneous differentiation into cells expressing endoderm markers, whereas pluripotency and neuroectoderm marker expression was systematically diminished.
CD68 marker expression was found in scattered subepicardial cells, and some of them co-expressed the Lyve1 antigen (Fig. 9f j).
The induction of stress marker expression was assessed by fluorescence microscopy after 48 h.
In this study, the surface marker expression was investigated on dental pulp stem cells (DPSCs), dental follicle cells (DFCs), periodontal ligament stem cells (PDLSCs), and buccal mucosa fibroblasts (BMFs) utilising surface markers for flow cytometry.
The viability, neurite outgrowth and neural marker expression was found to be comparable between the conventional poly-l-ornithine (PLO) surface and the CNT surface.
DCs were collected and cell surface marker expression was analyzed.
Activation marker expression was quantitated by flow cytometric analysis.
As a control marker expression was also evaluated in feeder cells alone (Figure 5A, B).
Colocalization of DiI label and cardiac-specific marker expression was examined and used to indicate CD of transplanted cells.
Surface marker expression was analyzed with a LSR I flow cytometer and by FlowJo software (Tree Star Inc., Ashland, OR).
The maintenance of ES cell/early epiblast pluripotent marker expression was also observed in cultures when FGF signalling was inhibited.
