Sentence examples for marker expression using from inspiring English sources

Cells were washed and stained for surface marker expression using fluorescent monoclonal antibodies.

An increasing number of recent studies evaluated hypoxia marker expression using TMA.

Cells were assessed for surface marker expression using fluorescent multicolor flow cytometry (FACSCanto II; Becton Dickinson, San Jose, CA, USA) as described previously.

EMT-induction was confirmed by morphological alterations and the detection of downregulated epithelial marker expression and upregulated mesenchymal marker expression using reverse transcription PCR.

Subsequently, we assessed surface marker expression using fluorescence-activated cell sorting analysis and found profound phenotypical differences between these differentiated cells.

For phenotyping analysis, CD4 and CD8 populations were identified using bivariate dot plots before assessment of homing marker expression using CD29 (β1 integrin chain) versus CD49d (α4 integrin chain) bivariate plots (see Fig. 8E and F).

Live cells were identified by 7-aminoactinomycin D (7-AAD) exclusion and analyzed for surface-marker expression using FACS Calibur (BD Biosciences).

Live cells identified by 7-AAD exclusion were analyzed for surface-marker expression using FACSCalibur or sorted using a Dakocytomation Mo Flo high speed flowcytometer.

To characterize the hES cell derived hepatic progenitor cells, we assessed the marker gene expression using immunofluorescence.

Analysis of marker gene expression using these HIFs indicated a negative correlation between the induced amount of transcripts of SA-dependent genes PR1, ICS and PR5, and the in planta bacterial growth in the HIF segregating at PRP-Ps2 locus, suggesting an implication of PRP-Ps2 in the activation of SA dependent responses.

Irradiated tumours showed reduced endothelial angiogenesis marker CD31 expression using immunoblotting (by 29.3±3.9%, Figure 6A).