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We found that changes in PMN CD marker expression required a higher concentration of anti-CII than ΤΝFα responses from PBMC.
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The exact effects and mechanism of IL-6 on lung cancer stem cell marker expression requires further investigation.
Using the luciferase reaction in vivo as a marker of gene expression requires that the substrate is nontoxic and well distributed to the animals' tissues after exogenously adding (usually via intraperitoneal injection).
With the exception of three genes (MMP12, S100A7 and SPP1) whose expression requires further validation, we were unable to find markers with potential utility for the detection of aggressive disease.
Each expression requires considerable thinking when drafting.
Second, mutant StC showed a delayed onset of Eya and of the MA33 enhancer-trap StC marker expression, indicating that TGFβ signalling is required for the expression of both markers (Fig. 3A,B,D,E).
RT-PCR allows for measuring marker expression in a highly sensitive manner but requires fresh samples and it too cannot provide a measure enzyme levels or activity.
Altogether, this suggests that TGF-β signalling is required to maintain key pluripotency marker expression in human EPI cells and a PE marker in vivo.
Cells from mouse and finch at this stage do not require ERK activation for the maintenance of pluripotent marker expression in vitro.
FACS data for cell surface marker expression was not obtained on 3D MC-bound cells because FACS analysis requires dissociating cells from the microcarriers.
However, further studies are required to better understand the discrepancy between Snail and other EMT-related marker expression after GC treatment.
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