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Un-stimulated NK cells did not affect the lysis of any of the EC types (Figure 7A C) and also did not change the cell surface marker expression pattern as shown in Figure 5A C.
The phenotypic marker expression pattern has also been reported to be associated with tumour aggressiveness in gastric carcinomas.
Indeed, marmoset adipose tissue-derived MSCs exhibited a similar marker expression pattern (K.E. and R.B., unpublished data).
Thus, instead of separating stemness marker-positive from negative cells, we studied the behaviour and marker expression pattern of pancreatic tumour spheres.
It has been demonstrated that different genetic pathways according to the phenotypic marker expression pattern of the tumour exist during gastric carcinogenesis (Morohara et al, 2006; Yamazaki et al, 2006).
When SNAI1 and TWIST1 were expressed in nontransformed HMLEs, Her2/ neu-transformed HMLEs, and H- rasV12-transformed HMLEs, the cells underwent EMT and acquired greater mammosphere-forming ability and a CD44+/CD24− cell surface marker expression pattern [ 11].
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H&E, IHC and IF analyses showed that in vitro cultured multilayered DE-DM CSs expressed appropriate tooth marker expression patterns including SHH, BMP2, RUNX2, tenascin and syndecan, which normally direct DE-DM interactions, DM cell condensation, and dental cell differentiation.
However, it may be difficult to yield reliable prognostic information for an individual patient from the expression pattern of EMT markers in surgical specimens; this is in part due to high variability of marker expression patterns in different tumor areas in a heterogeneous sample [127, 128].
Both compounds cause similar concentration-dependent changes in marker expression patterns.
The cells were analysed for the main lymphocytic, monocytic and erythroid marker expression patterns.
No significant differences in SYCP3 (meiotic cell marker) or PNA (acrosome marker) expression patterns were observed (Figure 4H).
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