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For fascin, this low- to high grade ratio of marker expression comprised 15% to 52% (P<0.001).
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This freedom of expression comprises three criteria.
Through a variety of experiments involving SCID mouse transplantation, using patient cells FACS sorted based on surface marker expression, the stem cell population was found to comprise of a primitive population with a CD34+CD38- phenotype.
Investigation of cell surface marker expression in bulk and tumorsphere-derived LA-N-5 cells revealed that tumorspheres are comprised of a more distinct subpopulation of cells expressing the drug efflux channel ABCG2, but the percentage of positive cells was only 2.9-fold increased, suggesting this is not likely to be a predominant mechanism responsible for the altered sensitivity.
Tumors, most commonly, tend to be comprised of multiple cellular clones and this fact may alter the marker expression.
TGF-β increased SMC marker expression on stiff substrates.
(B ) Quantification of marker expression.
In analyzing immunophenotypic marker expression in blast populations at diagnosis, more than half (25/48) of patients in this study had immunophenotypically distinct subpopulations as defined by their bimodal antigen expression, which often comprised more than 10% oftotal blast cells (Table 3, Figure 3).
Thus, co-expression comprises part of the positively correlated signal.
Keeping in mind that the CSCs with a high expression level of the CD44 marker (CD44high), comprised by heterogeneous population of CD44+ cells, possess the highest tumorigenic activity, they were isolated from a total EC population with a magnetic sorter (BDTM Imagnet).
After this filtering process, the marker dataset comprised 44 809 markers.
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