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Human primary osteoblasts were homogenously distributed inside 3D-ZTA and showed increased osteogenic marker expression compared to 2D-ZTA control.
Biocompatibility of the developed nSi Gel was confirmed by quantitative PCR and immunohistochemical tests which showed no significant differences in the level of inflammatory genes, microRNAs, and cell marker expression compared to the untreated control group.
The optimal polycistronic MGT vector improved both αMHC-GFP reporter expression and cTnT cardiac marker expression compared to separate Gata4, Mef2c, Tbx5 virus reprogramming (Wang et al., 2015a).
In response to Vmem depolarization, cells exhibited mostly decreased OS and AD marker expression compared to the normal trajectory of OS and AD differentiation.
Again, the optimized culture condition produced a statistically significant difference in maintaining mesoderm marker expression compared to other conditions.
Nonetheless, the granulocytes re-established by SPI1 exhibited typical morphology and surface marker expression compared with those from WT hESCs (Fig. 5e g).
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Aortic arches of p55−/−LDLR−/− mice showed approximately 50% less CD-68 macrophage-marker expression compared to controls, suggesting reduced presence of macrophages and thereby smaller plaques (Fig. 2D).
Moreover, after several further amplification (until 3rd passage) in hypoxic or normoxic conditions of the previously differentiated SMC, immunofluorescence studies showed that more than 80% cells continued to express SMCs markers whatever the cell environmental culture conditions with a higher contractile markers expression compared to control (aorta SMCs) signature of phenotype stability.
In the absence of TGFβ1, silencing CTGF or Snail1 alone did not drastically affect basal EMT marker mRNA expression compared with the NC group.
Furthermore, cells seeded on immobilized ALP/FS exhibited higher osteoblast marker gene expression compared to control FS.
Moreover, cells seeded on mineralized fibrin scaffolds exhibited significantly higher alkaline phosphatase activity as well as osteoblast marker gene expression compared to fibrin scaffolds and nHA incorporated fibrin scaffolds (0.25 and 0.5 g).
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