These results demonstrate that mMSCs cultured under hypoxic conditions classify as mesenchymal stem cells by cell surface marker expression and properly undergo tri-lineage differentiation.
Supplementary Figures 2 6 show key results (cell clusters, marker expression, and cell type assignments) in identifying the major cell types, endothelial, epithelial, mesenchymal, and immune sub-populations.
Retinoic acid treatment induced differentiation of glioblastoma stem cells, which led to the loss of CSC marker expression and the retardation of tumor growth through Notch signaling inhibition24.
The endocardium shares all these properties with arterial endothelium, including the arterial marker expression and exposure to the haemodynamic stresses and nitric oxide.
Depleting ILK blocked stiffness and hypoxia-dependent acquisition of CSC marker expression and behavior, whereas ectopic expression of ILK stimulated CSC development under softer or normoxic conditions.
BMP-7 released from PLAGA induced the muscle-derived cells to increase bone marker expression and form mineralized cultures.
Our results show that hypothermia exerts clear benefits on both RBC and leukocyte preservation for up to 72 h, accompanied by the maintenance of surface marker expression and inhibition of leukocyte activation (Fig. 1).
Notably, our achievement in stabilizing surface marker expression and blood counts for up to 72 h surpasses the recommended storage duration set by the WHO by three- and sixfold, respectively47.
(B) FACS histograms of p12 mMSCs grown in hypoxia+bFGF show that cells retain MSC specific surface marker expression and are positive for CD44, Sca1 and are negative for CD34, CD45, CD13, and CD31.
In this study, DC response to a set of 12 polymethacrylates (pMAs) was assessed in terms of surface marker expression and cytokine profile.
However, TGF-β increased chondrogenic marker expression and suppressed adipogenic marker expression on soft substrates, while adipogenic medium and soft substrates induced adipogenic differentiation effectively.
