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The analysis of differentiation marker expression also indicated that 5 µM genistein did not impair overall ES cell differentiation (Figure S2).
The association between Prnp and pluripotency marker expression also provides evidence of this contribution of Prnp to stem cell differentiation (e.g. the role of PRNP in neural cell differentiation [60]).
Screening for pharyngeal GFP marker expression also had the added benefit of distinguishing putative recombinant animals from wild-type animals that simply escape G418 selection, which has been demonstrated to occur at low frequency (Semple et al. 2012).
The TGF- β-induced mesenchymal shift was mainly detected in GBM cells with low or absent mesenchymal marker expression; however, GG7 GBM cells with already elevated mesenchymal marker expression also showed enhanced mesenchymal properties and elevated ZEB1 expression and migration following TGF- β treatment.
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It was remarkable that the combined-marker expression also showed prognostic value in TNM-stage IV patients.
As a control marker expression was also evaluated in feeder cells alone (Figure 5A, B).
The maintenance of ES cell/early epiblast pluripotent marker expression was also observed in cultures when FGF signalling was inhibited.
In addition to loss of marker expression, we also provide additional evidence to show that surviving epidermal ICs in foxi3a morphants lose their biochemical properties and function.
Efficient rescue of neural crest/placodal marker expression was also observed when Slug was activated at stage 11, but rescue was much less efficient when Slug was activated at stage 13 (FIG. 2; Table 2).
RPE de-differentiation has been shown to occur both in vitro [28] and in vivo [21] upon EMT marker overexpression and loss of TJ signalling components in PVR [35], [36] whereas EMT marker expression has also been linked with AMD [37].
Response scores and marker expression were also tested for association with outcome in a Cox regression model.
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