However, when a later marker of the endocytic route was used, CD63 characteristic of the phagolysosome, SO2 mutant showed poor colocalization with this marker, displaying a persistence phenotype as described previously by our group for murine macrophages [12].
Of interest, when results were re-analyzed using 'data from the matching normal DNA', the results were strikingly similar, wherein each marker displayed a sensitivity range of 85.1% to 95.6% for identifying MMR-deficient CRCs, and a specificity of 95.0% to 100% for detecting MMR-proficient CRCs.
Each marker displayed a varying degree of cross-species utility, possibly due to the differing degree of primer sequence similarity to chicken (Table 4, Additional file 3).
A cluster of syntenic FI-markers displays a chromosome-specific pattern of F2 genotypes, which is identical for all loci on the same chromosome and which displays the exact opposite pattern in all markers in repulsion.
In fact, both markers displayed a trend towards lower expression in hyperglycemic conditions.
The iPBS markers displayed a higher proportion of polymorphic loci (PPL = 92.5%) than the ISSR markers (PPL = 84.9%).
These markers displayed a high level of polymorphism overall (6.67 alleles/locus), though this was slightly lower within populations (3.10 4.88 alleles/locus).
The other significant markers displayed a low MAF (4 to 7%).
The discovered markers display a high level of specificity for the qPXO79_6 loci and could directly be deployed as foreground markers in marker assisted backcrossing (MABC) schemes.
