However, in their study, only one marker displays a difference in frequency up to 71%.
Of interest, when results were re-analyzed using 'data from the matching normal DNA', the results were strikingly similar, wherein each marker displayed a sensitivity range of 85.1% to 95.6% for identifying MMR-deficient CRCs, and a specificity of 95.0% to 100% for detecting MMR-proficient CRCs.
A clear signature of hitchhiking, however, will be seen if the marker displays a more balanced allele frequency distribution.
Each marker displayed a varying degree of cross-species utility, possibly due to the differing degree of primer sequence similarity to chicken (Table 4, Additional file 3).
Lastly, DG C and DG GP exhibited high r values between close markers, displaying a strong decrease with distance, with a faster decrease in C than in GP.
Glycerol-induced sporulation markers displaying a mutant sporulation phenotype (Phenotype), encoding proteins identified in glycerol induced spores (Protein), or associated with upregulated activities (Activity).
Markers displaying a segregation ratio greater than 3 1 for markers with an expected ratio of 1 1 and greater than 10 1 for markers with an expected ratio of 3 1 were excluded from map construction.
RADseq markers in testcross and intercross configurations are expected to segregate 1 1 and 1 2 1, respectively, markers displaying a segregation distortion (according to the criteria described in "Methods") were excluded from analysis.
The other significant markers displayed a low MAF (4 to 7%).
The iPBS markers displayed a higher proportion of polymorphic loci (PPL = 92.5%) than the ISSR markers (PPL = 84.9%).
