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However, it was impossible to pinpoint the causative polymorphism because of the low uneven marker density in that region in our panel (Fig. 5).
As tropical japonicas appear to be a potential source of interesting quantitative resistance alleles, this subgroup should be specifically explored through GWAS using larger panels and a higher marker density in a context of high blast disease pressure or using broad spectrum blast strains mixtures.
Furthermore, there were differences in marker density in different LGs.
Therefore, a suitable marker density in genetic maps is necessary for effective QTL mapping and MAS.
The marker density in this population was 40 markers per chromosome.
The marker density in the current reference Clementine map varied along the genome.
The marker density in the pericentromeric regions was high and abruptly dropped off at the border of the centromere.
The marker density in the current map is almost three times higher than that in the previous maps.
As a result, these four chromosome regions also showed a lower-than-average marker density in the consensus map.
Based on these results, nine additional families were genotyped and analysed to increase the marker density in regions of interest.
Differentiation of these three closely positioned QTLs is made possible by the improved marker density in this study and therefore finer mapping resolution.
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