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We confirmed the absence of recombination in these sensory cells by using antibodies against myosin VIIa, a cell type-specific marker (data not shown).
Tumor sections without tumor cell membrane staining did reveal stroma staining which was co-localized with a fibroblast-specific marker (data not shown).
More than 90% of the colonies recovered had undergone the appropriate recombination to remove the selectable marker (data not shown).
Target gene expression in pools of transfected cells was verified by Western blot and by flow cytometry using eGFP as a marker (data not shown).
Flow cytometry analysis of day 8 cultures revealed that over 90% of cells are positive for the HNK-1 marker (data not shown).
As shown with actin and Gp135 staining, and also with proper localization of ZO-1, a tight junction marker (data not shown), these cells were indeed polarized but failed to exhibit any polarization of flotillins.
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No human NSCs were detected in NSC mice by immunohistochemistry (IHC) for human specific markers (data not shown).
However, neither [TIMP-2]·[IGFBP7] (Fig. 2) nor individual markers (data not shown) discriminated well for AKI at the 18-h (pre-resuscitation) time point.
As an alternative, a downstream biological measurement such as the white blood cell count, C reactive protein, and procalcitonin was analyzed, but we did not find any significant relationship of IL-6 rs1800795 polymorphism with these markers (data not shown).
The cultures were negative for dopaminergic (TH), cholinergic (ChAT) and serotoninergic (serotonin) markers (data not shown).
Flotillin-1 and -2 also co-localized with uropod markers (data not shown).
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