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The most associated marker mapped at the distal border of this region, at position 628,091; more distally, the observed marker coverage was low, with only two additional SNPs detected in the panel under study, at positions 170,244 and 330,484 (Additional file 4: Figure S3); none of them was associated to bakanae resistance.
However, it has to be acknowledged that marker coverage was generally too sparse to detect subtle rearrangements.
The genetic map was built from 1,525 SNP markers, and it is therefore unlikely that insufficient marker coverage was the cause of short LGs.
The poorest marker coverage was found on P7, which had the fewest number of bins, total markers and the largest average marker interval.
At least for chromosomes 1A and 2A, the presence of gaps of marker coverage was reported in the consensus map of Marone et al. [ 19] and was found to correspond to the centromeric deletion-bins of wheat.
Taking into account the size of all LGs, the marker coverage was 2,664.36 cM for the female map, 1,305.58 cM for the male map, and 2,457.82 cM for the integrated map.
Similar(51)
CIM should ideally be performed after ensuring that marker coverage is sufficient to provide an unbiased estimation of background effects.
Therefore, using dense marker coverage is critical to detecting those effects.
Higher marker coverage is required in order to identify candidate genes more efficiently in diverse collections.
Molecular marker coverage is uneven among different taxa and between genomes.
Further genotyping, targeting the regions of low marker coverage, is being assessed in order to detect the presence of one or more additional QTLs, or potential modifier genes.
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