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If some as yet undiscovered heteroduplex repair system in E. coli could repair 18 nt loops, and if it preferentially repaired either Het I or Het II, reversing the marker configuration would control for this by switching which patch type (e.g. 5') is represented by which Het type (e.g. Het I), when the markers are reversed.
Using the marker configuration models, independent bead motion was detected in 5 of the high-migration cases, between the postoperative and 6-month follow-up examinations.
The marker configuration model RSA technique was used for measurement of the pose of a rigid body in situations where less than 3 markers could be detected in both images of an RSA radiograph (Kaptein et al. 2005).
The marker configuration model-based RSA technique (Kaptein et al. 2005) was used to solve the occluded marker problem in cases where less than 3 beads were matched in an examination at follow-up.
An increasing condition number indicates that the marker configuration approaches that of a straight line, which may have any direction, and the condition number has been shown to explain as much as 92% of the variation in overall rotation (Ryd et al. 2000).
Marker configuration models were also used to test for bead loosening and possible deformation of the trabecular metal base plate.
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To minimize multiple testing, we did not analyze any further marker configurations.
One hundred and seven primers produced 20 marker configurations (Additional file 4: Table S4, Additional file 5: Figure S2).
This can be due to missing marker data, but also to random estimation errors in the recombination frequency inherent to the marker configurations [ 80].
Among them, 69 primers follow disomic marker configurations but 38 primers (35.5%) do not fit disomic configurations, producing more than 3 amplicons in one or both parents (Additional file 4: Table S4).
Model-based and marker-based RSA methods have different precisions for different prosthetic designs and possible marker configurations, and comparison between studies using different models is therefore problematic (Baad-Hansen et al. 2007, Lorenzen et al. 2013).
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