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We determined mean residence time (MRT) and peak marker concentration using the original profile observations and regressions.
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For the vast majority of the ruminant samples, an increase in the host-associated marker concentrations using either of the three ruminant markers corresponded directly to an increase in concentrations of the AllBac marker.
An agarose gel evaluation is sufficient to check the DNA quality (i.e., nondegraded) and concentration using a suitable marker (e.g., Marker II; AppliChem GmbH, Darmstadt, Germany).
The log2 transformed marker concentrations were used as predictors on a logistic regression model against the outcome (healthy vs PDAC).
Research is needed to investigate the mode of action for As effects and assess human health effects of As at low concentrations using biologic markers.
Total intestinal Bacteroidetes marker concentrations were enumerated using the AllBac assay.
Effects of this therapy were assessed using serum marker concentration, Ki67 and gastrin-immunoreactivity of the primary tumour.
Multi-parametric models for combinations of markers were evaluated using log2 transformed marker concentrations as predictors on a logistic regression model against the outcome (healthy vs PDAC).
We found the quantification of PCR product problematic because the nanodrop overestimated the concentration and using quantitative markers on polyacrylamide gel seemed to underestimate the concentration.
Receiver operating curves (ROC) were used to determine if serum marker concentrations discriminated between cases and healthy controls [29].
BSA levels in BALF supernatant were used for normalization of probable expressed local marker concentrations, and the [local marker]/[BSA]-quotient was calculated.
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