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Brazilin and protosappanin B are two major bioactive homoisoflavones isolated from C. sappan, which are used as the marker components for the quality control of the crude drug in the current version of China Pharmacopoeia [11].
Calibration curves of eight marker components (n = 3).
Calibration curves of the five marker components showed good linearity with correlation coefficient (r) ≥ 0.9998 in the different concentration ranges.
We prepared two batches of HRT, fHRT, HRT-BU, and fHRT-BU under the same condition and analyzed marker components in each batch by HPLC analysis.
The chemical standards used to identify and quantify compounds in the SSTW were as follows: liquritin, glycyrrhizin and baicalin (marker components).
To distinguish the difference of components between non-fermented and fermented HRT, we characterized the seven marker components in HRT by HPLC analysis.
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The marker component of pEnt-Emr/Zeo was amplified using long-range PCR with the forward and reverse primers containing 50 nt of homology sequence (Table 2A).
Standard solutions were prepared by dissolving each marker component in 100% methanol at 1 mg/mL.
In addition, cytosolic localization displayed for HydE, a marker component of hydrogenase maturases.
In this study, we found that fHRT shows a change of the marker component profile compared to HRT.
Because a marker component of the maturases HydE is localized in the cytosol as well, we can confidently predict that maturation of hydrogenase takes place in the cytosol of N. gruberi.
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