Increasing concentrations of the detergent digitonin solubilized to comparable levels the OMM of Opa1 −/− and Opa1 −/−::OPA1 mitochondria loaded with NBD-cholesterol, as revealed by the disappearance of the OMM marker TOM20 and the enrichment in the IMM marker complex III.
Using transfected COS7 cells, we found minimal overlap with endogenous GM130, a marker of the Golgi complex in all transfected cells (Fig. 7C); however, the bulk of the LRSAM1 labeling was in a perinuclear compartment surrounding the GM130-positive Golgi.
Alberge et al. tested this idea by studying the localization of a respiratory complex called nitrate reductase which is important for generating energy in the absence of oxygen through the introduction of a fluorescent marker tagged to the complex in the cell membrane of a rod-shaped bacterium called Escherichia coli.
While ceramide conjugates are typically used as markers for the Golgi complex in other systems [48], [49], we observed that SNARE2.1 co-localized with BSA-conjugated BODIPY-ceramide in T. cruzi.
Immunohistochemistry for EMT markers and E-cadherin/ β-catenin complex in 134 patients with pulmonary NETs between 1990 200909.
The less in numbers but more significant in terms of functions has made the nsSNPs an ideal marker type in complex trait association analysis.
The Indian Genome Variation Consortium (IGVC) project, an initiative of the Council for Scientific and Industrial Research CSIRR)—was set up to develop a database of genomic variations in Indian population for predictive marker discovery in complex diseases such as diabetes, asthma, neuropsychiatric, infectious and cardiovascular disorders, response to drugs, etc. (4).
Currently, Alus are repeat-masked in all studies pertaining to identification of predisposition markers in complex disorders.
We discuss the advantages of the proposed eSPR approach for the development of highly selective sensor devices for the rapid and reliable detection of multiple molecular markers in complex samples.
This method greatly facilitates the quantification of chemical markers in complex matrixes with only a small amount of sample.
