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No other marker combination was able to improve on the predictive power of this model.
However the suggested proteins for the peaks with best marker combination was chosen for confirmation of method and validation in an external sample set.
Perhaps because their effect is harder to replicate, only one epistatic marker combination was present in as much as 5 samples, while 2 combinations were present in 4 samples, and three combinations in three samples.
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The necessity for marker combination is shown not only by lack of specific staining of the nephrogenic mesenchyme but also by high percentage of expression of single markers in HFK cells, over 50% of cells for markers such as CD24 and CD133, as well as the relative high marker abundance within the EpCAMbright fraction and persistence in high levels in the HAK.
Overmeer et al. demonstrated that this marker combination is optimal for detection of CIN3 lesions [ 69].
Three different marker combinations were examined for performance in phylogenetic reconstruction: rbcL+matK, rbcL+trnH-psbA, and rbcL+matK+trnH-psbA.
All marker combinations were tested.
Of the conditional results, thirteen marker combinations were consistent in 8 samples.
Promising marker combinations are identified in the training sample, but more reliable FPR and TPR measurements are made in the test sample because it involves different data.
The means of segregants containing various marker combinations were compared, and the difference between the means was used to estimate trade-offs.
The most promising marker combinations were CEA + IL-8 reaching 37% sensitivity at 83% specificity and CEA + CRP with 35% sensitivity at 81% specificity.
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