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Sparcl1 is known as an astrocyte marker colocalized with Gfap [24] and is also known as a candidate gene for multiple sclerosis [25].
Again, the activation marker colocalized with mH2A2 and was more abundant in summer, whereas the repression marker colocalized with mH2A1, and only during the winter season.
Adducin, a predominantly postsynaptic marker, colocalized with and surrounded the presynaptic membrane defined by HRP staining (Fig. 3A).
OLIG2, a well-known oligodendroglial nuclear marker, colocalized with TPPP, demonstrating that some of the TPPP immunoreactivity is in the oligodendroglial nucleus.
As shown in Figure 3, primary cultures highly enriched in HCs exhibiting positive immunoreactivity for Prox-1, a typical nuclear HC marker, colocalized with the fluorescence for Opn4x.
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Lysosomal-associated membrane protein 1 (LaMP1), a lysosomal vesicle marker, colocalizes with quinacrine, a specific ATP-combining chemical, in primary Schwann cell granules in culture, thus indicating that ATP is stored in lysosomal vesicles [ 33].
FYVE domain marker colocalized also with RabF2a and RabF1, today recognized as multivesicular or late endosomal Rab GTPase markers [ 32, 33, 40].
The positive MΦIL-4 marker CD200R showed a low expression and low colocalization with CD68, whereas the negative MΦIL-4 marker CD14 strongly colocalized with CD68.
As shown in Fig. 2C, GFP (a marker of transfection) colocalized with low levels of ABCG2 staining demonstrating that transfection with shABCG2-3 downregulABCG2ABCG2 expression in these mouse ES cells.
We found that vesicular glutamate transporter (VgluT), a marker of glutamatergic neurons, colocalized with r-opsin1, a marker of eye photoreceptors (Arendt et al., 2002; Randel et al., 2013; Figure 3 figure supplement 2, Figure 3 figure supplement 3), indicating that the photoreceptors are glutamatergic.
In addition, colocalization studies with pre- and post-synaptic markers revealed that Atp6ap2 colocalized with several presynaptic markers, including synaptophysin (Supplementary Material, Fig. S5G), VGLUT1 (Supplementary Material, Fig. S5H) and VGLUT2 (Supplementary Material, Fig. S5I), and only poorly colocalized with PSD95, a postsynaptic marker (Supplementary Material, Fig. S5F).
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