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The details on SSR marker code, source species and number of SSRs developed are given in the Table 1.
The marker code of the polymorphic SLAFs was analysed according to the population type CP (cross pollinators), which consist of five segregation types (ab × cd, ef × eg, hk × hk, lm × ll, nn × np).
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Each marker (coding or non-coding) is then classified using its level of conservation.
A human marker (coding or non-coding) conserved only at between human and opossum is labeled as metatheria marker.
Therefore, it is assumed that the recombinational landscape presented in this paper should not be biased by the type of marker (coding versus non-coding) used for linkage analysis.
The yellow circle frames represent marker codes, and the blue square frames represent cultivar codes.
Cultivar and marker codes are shown in Tables 1 and 2. By using five core markers, 12 prevailing cultivars could be identified.
The T-DNA region of the binary vector contained a selectable marker coding for the hygromycin phosphotransferase (hpt II) gene under control of CaMV 35S promoter (provided by Dr. Su-May Yu, Academia Sinica, Taipei, Taiwan).
This marker codes for a key enzyme controlling cellular oxidative stress-glutaredoxin (thioltransferase).
The parent strain was CUY1147ΔLEU2 (CUY1147 (Kosco et al., 2001) in which the LEU2 gene was replaced with a marker coding for G418 resistance).
Each integer represents the genotype for one individual at one marker, coded as 0 for missing, 1 for one homozygote, 2 for the heterozygote, and 3 for the other homozygote.
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