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To minimize this problem in the present study, marker clusters were used instead of single markers (Cappozzo et al., 1997).
Genotypes in the form of marker clusters were checked manually in the MassArray TyperAnalyser software.
Moreover, the marker clusters were observed in all of the LGs (Fig. 2 and Supplementary Table S3).
Marker clusters were associated with regions of segregation distortion, such that they were composed of either all distorted markers or all non-distorted markers.
Marker clusters were arranged to decrease the possibility of contact between the clusters during movement and to avoid overlap and mislabeling during the analysis (Fig. 2c).
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The second finding of this study reveals that a common thread of the identified cancer marker clusters is the presence of both agonist and antagonist members within the cluster.
The acromion marker cluster was made of an alloy triangular base specifically shaped to be positioned over the flat part of the acromion (Cereatti et al. [2014]).
In particular, while the arm was hanging along the side of the torso, this position with respect to the scapula marker cluster was identified as the baseline neutral position.
The genetic length of a marker cluster was regarded as half the sum of its genetic distances to the two adjacent marker clusters (see also Table S7).
The physical length of a marker cluster was estimated by summation of the lengths of the scaffolds or scaffold regions with markers on it that mapped in the marker cluster (see also Table S7 and Table S12).
Some dissimilarity in RAD marker clustering was also observed between the two families.
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