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Figure 2 Typical RRLC-QQQ MS/MS chromatograms of marker chemicals in Shen-Fu decoction (A) standard mixture (B) Shen-Fu decoction.
Using an optimized elution gradient, the main components were separately eluted within 11 min. The typical RRLC-QQQ MS/MS chromatograms of the marker chemicals in Shen-Fu decoction are shown in Figure 2.
By using RRLC coupled with an ESI triple quadrupole tandem spectrometer, we developed and validated a rapid, simple and reliable method to simultaneously determine ten marker chemicals (ginsenoside Re, Rg1, Rb1, Rc, Rb2, Rd, Rf, aconitine, hypacoitine and mesaconitine) in the Shen-Fu decoction.
In some waters, THMs could be used as marker chemicals for the whole mixture but this should be verified through chemical analyses of different compounds in the water.
Assigning an unambiguous term to these chemicals is complicated by the fact that in some NPs, chemicals many have been determined to be "pharmacologically active", while in other NPs, in the active chemicals are unknown and so product content is standardized to marker chemicals.
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The marker chemical is composed of a fluorescent dye and an antibody that sticks to specific targets.
Patients had been given a marker chemical called bromodeoxyuridine (BrdU), which is incorporated into the DNA of dividing cells, to follow the growth of their tumors.
Additionally, associations with marker chemical pollutant(s) are not well validated.
A smoker's score in the Fagerström test correlates with the salivary cotinine levels and is a marker of chemical dependence[ 26- 28].
Permanent markers contain chemicals that aren't meant to be used on skin.
His breakthrough came when, after hearing a talk about epigenetic markers -- chemical modifications that affect gene expression -- he tried to block epigenetic mechanisms in his assays on the chance that these might be underlying his failure to induce the retrotransposon's expression and mobility.
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