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Besides the intensity profile vector x i, also a particular retention time (rt) index and mass-to-charge ratio (m/z) is associated with each marker candidate i in a given sample.
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Furthermore, eight clusters only contain a single marker candidate.
The statistical comparison of the single urinary peptides and proteins in the discovery data set resulted in the tentative identification of 222 potential marker candidates (see Table S3/set I).
After taking repetitive sequences into account, there were 1497 non-redundant SSR marker candidates including 24 class I SSRs (≥ 20 bp) and 1455 class II SSRs 12-199 bp).
70 proteins including HPX, CTTN, CDH1, EGFR, MUC1 were found as LPIA-type marker candidates, 15 protein candidates for MIA-type marker included CRABP2, LMO7, and RNPEP, and 26 protein candidates for AIS-type marker included LTA4H and SOD2.
The processing resulted in 6048 marker candidates.
AF provided marker candidates from DMH studies.
We also suggest several marker candidates valuable to investigate further.
P was a matrix of principal component vectors, S was the vector of genotypes at the candidate marker, and I was an identity matrix.
Forty eight candidate class I SSR markers were identified and 24 of these were initially screened for polymorphism between the parents of two RIL populations yielding nine and 14 novel SSR markers for the cv.
"While we lost in terms of implanting a legal marker, I do think a cultural marker has been made," he said.
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