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Pzp200bar also contains the BASTA resistance marker (bar gene), which permits the selection of transformed plants.
A second marker, bar, tested at two other subtelomeres, was similarly sensitive to nst-1 function.
The marker bar M1 was set to indicate cells with high Rh-123 efflux.
The 1.1 kb cre coding sequence was cloned into the multiple cloning site of pCAMBIA3300 along with herbicide resistant marker bar and named as pBK16.2 (~10.6 kb).
The former encodes the Barnase gene that give rise to male sterility, which is replaced in the latter by the Barstar gene which restores fertility: both also have the selectable marker bar which confers tolerance to the herbicide glufosinate ammonium.
Marker bar M2 was set to indicate the cells with low Rh-123 efflux.
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The blue (autosomal marker) and cyan (X-linked marker) bars in Figure 3 for the case of a single X-linked factor (X on the abscissa) illustrate this effect.
Elimination of the cre gene (and of the associated selectable marker- bar) was accomplished by genetic segregation, thus obtaining completely marker-free mustard plants expressing ASAL.
To determine whether telomeric silencing occurs at other Neurospora telomeres and works on other genes, we inserted the selectable markers bar, encoding Basta resistance, and ad-3A proximal to two other telomeres, namely those of chromosome arms VIIL and IIR.
The layout bars (or mark bars) can be marked with a soapstone marker, a paint pen, a piece of lumber crayon, or with spray paint.
Notably, our previous analysis indicated that the mtDNA divergence could not be explained by mtDNA introgression, nor did it correlate with possible gender-biased dispersal differences, which are common phenomena in many instances of distribution discordance between mtDNA and nDNA markers (Bar-Yaacov, Arbel-Thau, et al. 2012; Toews and Brelsford 2012).
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