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Graphene was transformed to an electrode chip, which can be used as a marker as shown in Fig. 6a.
The improvement allowed gene manipulation using the reusable pyrG marker as shown by the restoration of laeA-mediated aflatoxin production in an A. flavus laeA-deleted mutant.
Gene disruption was carried out by homologous recombination, and we confirmed that creA was replaced with the pyrF marker, as shown in Figure 2.
To make isogenic strains, isolates were tagged with an antibiotic marker as shown in Figure 1B.
We prepared a positive selection marker and a negative selection marker, as shown in Figure 1.
Five zones centered either on the tip, the nuclear region or the cell wall region were defined along the apical and sub-apical protonemal cells expressing a fluorescent organelle marker as shown in figure 1A.
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This removed a total of 58 SNP markers as shown in Additional file 5: Table S3 (Online Resource 3).
To facilitate the collection of accepted and rejected gaps, we first manually drew a few lines from road markers as shown in Fig. 2.
In contrast, clonogenic BCs do not express other bronchial or alveolar lineage markers as shown by RNA-Seq and confirmed by immunostaining (Figs. 1F and S1B).
Nevertheless, some cells in the hypothalamic neurons cultures kept the expression of multi-potency markers as shown by the immunostaining for SOX-2 marker (Figure 4-C).
Furthermore, both wt and EcadAb ES cells did not express pluripotency markers as shown by RT-PCR analysis (Figure 5B(i) and (ii); labelled pluripotency).
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