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The aliquots containing any BAC ID positive for an STS marker are scored as positive ( = 1).
Clones with two or more abnormal microsatellite marker are scored as MSI-high (MSI-H), while MSI-low clones have only one unstable marker (Boland et al., 1998; Haydon and Jass, 2002; Merok et al., 2013).
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Different sizes of one marker were scored as different alleles.
The data for each marker were scored independently by two researchers.
Immunohistochemical staining for each marker was scored by one pathologist (R.Q).
Each marker was scored as previously reported (Galgano et al, 2006).
Each allele of an SSR marker was scored as a dominant marker (present = 1 and absent = 0).
Each marker was scored individually for each line and compiled into a single excel file based on parental segregation data.
The expression level of each marker was scored based on the positive percentage by stained cells by clinical pathologists.
The CK5/6 marker was scored as positive when weak or strong cytoplasmic and/or membranous staining was visible and otherwise scored as negative.
When the intensity of a band at a locus was too weak to assume its presence, the marker was scored as missing data.
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