Additionally, a recipient strain of Synechocystis lacking all three psbA genes was produced by a combination of traditional marker replacement and markerless replacement techniques.
This sort of vision-based tracking can be classified into two groups namely marker-based and markerless methods [19].
Single-deletion strains as to each candidate region were prepared using a λ Red recombination system and negative selection marker sacB (markerless deletion method) previously reported (Supplementary Fig. S1, Supplementary Table S1).
To realize these functions, we developed about 3D face set representation of environment converting from point clouds obtained 3D camera, robust and markerless known object recognition using Improved LINE-MOD, and motion teaching method to 3D robot model using interactive markers.
Due to its thermo-sensitive origin of replication, the plasmid can be eliminated at non-permissive temperatures, and markerless deletion mutants can be obtained.
Also of interest is providing a range of rendering possibilities covering image-based rendering, virtual actors and markerless motion capture methods.
In the context of the recently introduced highly versatile and markerless recombineering techniques [9] [12], [15], this method eliminates the last hurdle to perform virtually any kinds of imaginable targeted BAC modifications.
These plasmid-cured mutants were unable to grow in liquid medium supplemented with erythromycin, demonstrating efficient plasmid curing and markerless mutagenesis.
Chiang et al. modified the conditional-replication, integration, and modular plasmid system produced by Haldimann and Wanner [ 28], and developed a replicon-free and markerless method (RMM) for the chromosomal insertion of genes [ 26].
Insertion of antigen expression cassettes by recombineering additionally circumvents the requirement for a reporter gene or selectable marker and its removal by transient-dominant selection [70] when a markerless product is required.
