For cultivation of the different recombinant C. glutamicum strains carrying the pEKEx2- Lbadh plasmid, a single colony of each strain was inoculated into 10 ml BHIS medium (37 g l−1 brain heart infusion, 91 g l−1 sorbitol) containing the appropriate selection marker as described above and grown overnight at 30 °C and 120 rpm.
About 90 seedlings from each of the progeny lines were genotyped with a marker on the heterozygous region and grown in the greenhouse for phenotypic assessment.
All carry the plasmid marker and all grow at 30°. Figure S3 shows drop growth tests for four clones each of the ipl1 -2 and ipl1 -2 duo1 -76 and ipl1 -2 ndc80 -8 genotypes.
This suggests that revealing or discussing one's HIV serostatus is a marker of intimacy and growing trust between partners [ 1, 14].
As part of an ongoing research project, we searched for the presence of cells positive for known stem cell markers in pedicles and growing antlers of fallow deer (Dama dama) [24], [25].
The CNS-1, U251 and GL261 models, in comparison, share several histological surface markers with human GBM and grow in a very similar pattern (i.e. diffusely infiltrative) in vivo.
In addition, cells sorted for cancer stem cell markers and cells growing as spheroids were significantly more resistant to chemotherapeutic agents than were parental cells, and were highly tumorigenic in mice.
It had been reported that a side population from mouse anterior pituitary is enriched in stem-like cells that express stem/progenitor cell markers, but not differentiated cell markers, and can grow in floating spheres in culture (Chen et al, 2005).
WT and PS1-hp mutant strains containing either pDK243 LEU2 (1X-ARS site) or pDK368-7 (8X-ARS sites) with a LEU2 marker were grown in synthetic complete media leucine to stationary phase, then plated onto YEPD and synthetic complete media leucine to determine plasmid stability under selection.
Cells carrying the natMX4 marker were grown on YPD containing 75 µg/ml nourseothricin (Jena Bioscience, Germany).
