We generated double-heterozygous transgenic flies that harbor the xbp1-EGFP marker and express the wt-MYOC and each of the four mutants (R342K, Q368X, D380N and K423E) using the gmr-Gal4/UAS system [21].
We found in our model that the most powerful DCs that stimulate T cells were also positive for the Ly-6C and F4/80 markers and expressed MHC II strongly (Figure S5B).
A fourth, double-negative population - dubbed stromal - lacked epithelial markers and expressed PDGFRβ (CD140b).
The outgrowths generated were positive for both luminal and basal markers and expressed the appropriate genotype (Additional file 9D,E).
We next studied the gene and protein expression of cardiac markers, finding that zebularine-treated cells highly expressed cardiac-restricted markers and expressed low levels of pluripotency factors.
The evaluation, annotation and classification of genomic markers and expressed transcripts would be of great utility for both functional genomics and systems biology research in plants.
Conversely, those cell lines which were relatively insensitive to erlotinib lacked those epithelial markers and expressed proteins characteristic of mesenchymal cells, including vimentin, fibronectin and Zeb-1 and exhibited a more fibroblastic, scattered morphology.
In this work, we proved that Pax6 cells did not colocalize with the glial marker GFAP and expressed the neuronal marker HuC/D.
