EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass.
The BG8-3 and BA1-12 end sequences were then used to design several contiguous markers and allowed the identification of a BAC clone (HD6-1) bridging the gap.
RNA-seq on two map-cross larvae provided a reference transcriptome that identified nearly 1000 mapped protein-coding markers and allowed genome-wide analysis of conserved synteny.
The marker intervals for this group were larger than those of the Rattus Chr 10 markers and allowed us to map 83.0 Mb (~82%) of Rattus Chr 14 in the deer mouse with only a few markers.
They compared these results to their split-GAL4 collection, which validated the marker lines and allowed them to fill in gaps.
Heterozygous recombinant sub-NILs for the target QTL regions were marker-selected and allowed to self-pollinate, and recombinant homozygotes were marker-selected (BC6S2 generation).
Kohlmann, A. et al. Gene expression profiling in AML with normal karyotype can predict mutations for molecular markers and allows novel insights into perturbed biological pathways.
It harbors kanamycin and hygromycin-resistance markers and allows the constitutive production of N-terminal His6-tagged proteins in mycobacteria under the control of the phsp60 promoter.
We compared allele frequencies between the PRIMO and HIV controller patients using an algorithm dedicated to association studies using bi-allelic markers and allowing fast computation of unbiased exact P-values[47].
