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We thus performed a multiple marker analysis with haplotypes (at least 5% frequency).
These cDNAs were used for MSCs marker analysis with PCR.
We identified single nucleotide polymorphisms (SNPs) in each gene and carried out single SNP marker analysis with the photosynthesis parameters in our segregating population.
Next, to examine whether the mis-localized β-gal-positive cells possess NE or SE character, we performed a marker analysis with immunohistochemistry (Fig. 6D E′).
Results show that penalized methods outperform single marker analysis, with the main difference being that penalized methods allow the simultaneous inclusion of a number of markers, and generally do not allow correlated variables to enter the model, producing a sparse model in which most of the identified explanatory markers are accounted for.
Marker analysis with the basal marker CK5 and the lumenal marker CK8 showed that AcP10-positive tumor cells, like their normal counterparts, co-express CK8 (Fig. 6); thus loss of Pten function did not influence the cell type in which AcP10 was expressed.
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Since we were more interested in the R allele (band 1), only two genotypes were grouped based on marker analysis; genotypes with the band 1 and 3 were scored as "h" and those with band 3 and 2 as "a".
QTL mapping was performed in R/qtl version 1.27-10 (Aretds et al. 2010) using single marker analysis combined with interval mapping, resulting in simple interval mapping (SIM) analysis.
For surface marker analysis, cells stained with Hoechst 33242 were further incubated (30 min at 4°C) with anti-CD29-APC (1∶100), or anti-CD44-APC (1∶100; BD PharMingen) and then stained with propidium iodide (1 µg/ml) before analysis.
We performed a NEO single marker analysis on markers with an FDR < 10% in the behavioral QTL mapping.
JD explained DRC results to some participants, performed ancestry marker analysis and helped with DRC assay internal validation.
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