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Faecal sampling was initiated immediately after marker administration and continued for 120 h.
All marker administration was performed under medical supervision, with the patients receiving continuous cardiovascular and respiratory monitoring.
Blood samples were then taken via the intra-arterial cannula at the following time points after marker administration: 5, 10, 15, 30, 60 and 120 minutes and 4, 6, 12 and 24 hours.
Endogenous fluorophores are thus acting as intrinsic biomarkers, and their AF properties can be exploited as diagnostic parameters for a direct characterization and monitoring of physiological or altered morphofunctional tissue properties, in the absence of perturbation due to exogenous marker administration.
Study participants had to be aged ≤60 years, have an expected ICU length of stay >24 hours, evidence of a systemic inflammatory response syndrome [ 10] in the 24 hours prior to marker administration, a plasma CR concentration <120 μmol/L and no history of chronic kidney disease (CKD) or renal replacement therapy.
Marker administration was well tolerated, with no adverse events reported.
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