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Cell marker activity was compared between ES cells and mouse embryonic fibroblasts (MEFs).
When using scaled-up preparations of isolated acidocalcisomes by our previously published protocol [2], the acidocalcisome fraction showed a high level of contaminating mitochondria (Fig. 2B), with greater than 50% of the mitochondrial marker activity, succinate-cytochrome C reductase, co-purifying along with AMDP-sensitive pyrophosphatase (Fig 2A) [21], the acidocalcisome marker [1].
Such unique expression patterns are likely attributable to the effect of the selection marker activity on the overall cell physiology.
Clearly, yield and enrichment calculations become less dependable when they are based on a tiny fraction of the marker activity.
With these modules, we first asked how the selection marker activity is affected by the relative arrangement and the activity of the gene expression cassette.
In contrast to that, adding 15 to 25 mg silver powder showed a marked decrease in marker activity compared to titanium alloy control [ 11].
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Sucrase (EC 3.2.1.48, enterocyte apical membrane marker) and sodium-potasium-ATPase (EC 3.6.1.3, enterocyte basolateral membrane marker) activities were determined in BBMV suspension [ 2].
Third, transcriptional profiling and expression analyses have shown that cambium marker activities are barely reduced in wox4 mutant plants [ 47, 52].
The myocardial infarct area, oxidative stress markers, activities of myeloperoxidase (MPO) and creatine kinase (CK) were determined after the experiment along with the assessment of the effect on apoptotic activity by using DNA fragmentation analysis.
Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages.
The protective effects of pomegranate on serum sex hormones, oxidative markers, activities of antioxidant enzymes and histopathology of testes were determined in CCl4-induced reproductive toxicity in rats.
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