Excitatory neuron loss in these sheep is markedly regional, localized early reactive changes accurately predicting neuron loss and subsequent symptom development.
However, HA-tagged Bcl-xL differed markedly, being localized almost exclusively to the mitochondria with little evidence of cytosolic expression.
For example, transgenic mice expressing only VEGF120, an isoform lacking HSPG affinity [ 23], show a shallow VEGF gradient in the developing hindbrain [ 13] and retina [ 12], whereas wildtype mice, which predominantly express the heparin-binding VEGF164, have a VEGF spatial distribution that is markedly more localized.
This was supported by the immunocytochemical results that NR1, NR2A, NR2B, PSD-95 and synaptophysin are markedly co-localized in the HDC neurons (Figure S4A).
The Tn antigen levels markedly increased and localized in intracellular puncta and in the SG lumen at stage 12 and 13.
Ghosh et al. showed using IHC that overexpression of underglycosylated MUC1 localized markedly in normal breast tissue adjacent to tumors [ 29].
Skin biopsies from patients with scleroderma were characterized by markedly elevated Nab2 expression, localized primarily in the nuclei of epidermal keratinocytes and cells lining dermal appendages.
Despite high sequence conservation among distinct NL isoforms [ 2, 4], the subcellular distributions of NLs differ markedly: NL1 is predominantly localized at excitatory synapses, whereas NL2 exclusively localizes at inhibitory synapses [ 17– 17].
We have demonstrated that EPR spectra of these spin-probes localized in the thylakoid lumen markedly change with the light-induced acidification of the thylakoid lumen in chloroplasts.
The number of synapses localized at spines was also markedly reduced in DS cocultures (Figure 7D).
