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In contrast, the mRNA signal corresponding to endogenous p21 bearing the full-length 3'-UTR was markedly less stable in IL-3-deficient medium than with physiologic IL-3 (Figure 1D).
As expected, the cruciform structure was markedly less stable, with an average difference 23.3 +/− 0.2 kcal/mol.
Furthermore, in Figure 1d we see that the phosphosite mutants are markedly less stable than wild-type ΔNp63 α, with the triple mutant exhibiting least stability.
Assays showed that the stability of RCs in SMA/lipid nanodiscs is comparable to that of RCs in intact membranes or in DDM micelles at temperatures between 40 and 70 °C, but that RCs in LDAO micelles are markedly less stable.
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