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Both compounds markedly induced expression and protein levels of MMP-9, the induction being significantly lessened upon using an anti-TLR4 antibody or TLR4 siRNA, or the inhibitor (parthenolide) of NF-κB (Fig. 7).
To examine this hypothesis, we randomly analyzed OsWRKY13 binding activity to the promoters of Os06g15430, Os07g33710, and Os04g27100, which showed markedly induced expression in OsWRKY13-activated plants and a tendency of reduced expression in OsWRKY13-suppressed lines and their promoters also harbour W-boxes.
Nevertheless, PL (0.5 and 1.0 mg ml−1) markedly induced expression of a cyclin-dependent kinase inhibitor p27.
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In this way, TCDD and other AhR agonists markedly induce expression of the drug-metabolizing enzyme cytochrome P-450 (CYP) CYP1A1, which is commonly considered as a paradigm of AhR gene targets and known to detoxify and to bioactivate carcinogens [1].
HSP70 expression was weak before heat treatment, but heat shock markedly induced HSP70 expression.
PHA markedly induced CD69 expression both on CD3+ and CD25highCD127low T cells (Figure 4b).
Real-time quantitative PCR and Western blot demonstrated that Egr-1 markedly induced Rad expression at both mRNA and protein levels (Figure 3), which is consistent with Egr-1 transactivation of the Rad promoter.
As shown in Figure 5e, BA markedly induced the expression of PPAR β/ δ in BV2 cells.
TNF-α stimulation markedly induced the expression of ICAM-1 protein on the membrane of the endothelial cells.
As shown in Fig. 2 A, treatment of VICs with TGF-β1 (5 ng/ml for 48 h) markedly induced αSMA expression when compared with untreated controls (dashed line).
Supporting these findings, in transfected HEK293 cells ANP, via GC-A/PKG I, markedly induced the expression of RGS2 and its translocation to the plasma membrane.
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