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Figure 2A shows that, compared with vehicle control treatment of A431 cells following blockade of new protein synthesis with cycloheximide, treatment of the cells with 1, 9 PA markedly expedited the degradation of HIF-1α; the degradation time was shortened from 60 min in the control cells to less than 20 min in the cells treated with 1, 9 PA.
We present a side-by-side comparison of all steps and report an integrated experimental system that greatly streamlines and expedites the entire procedure while markedly reducing its associated costs about 250-fold compared to conventional phage display selection.
In addition, linking the A2UCOE upstream of CMV promoter driven cassettes prevents transgene silencing and markedly increases median levels of expression in stably transfected cells, thereby substantially expediting the isolation of lines for the manufacture of therapeutic proteins.
"Expedite," he would say.
Both would expedite service.
Governments should expedite the process.
"We will expedite their repatriation".
You expedite this, you expedite that, divorce papers, whatever else".
"We want to expedite everything.
Did Cronkite expedite Kennedy's decision?
He had changed markedly.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com