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All miRNAs selected based on differential expression were verified as markedly differentially expressed, with p-values varying from 10-7 to 10-21 and FC values up to 95 (Table 3).
It is also worth noting that the gene encoding the IkappaB inhibitor was up-regulated during infection with A. fumigatus, despite of the fact that NFkappaB itself was not markedly differentially regulated.
Of these, 23 miRNAs including 16 known and seven novel miRNAs were markedly differentially expressed with an absolute value of log2 ratio (Na200/CK) > 10.
20– 25 The majority of these were more markedly differentially expressed in ACPA-negative than ACPA-positive RA (figures 3A,B and online supplementary figures S4A C).
Among the genes that were markedly differentially expressed in the 3 tetrapod species, an OrthoMCL group corresponding to the Tribbles gene family was identified.
Among the 103 upregulated and the ten downregulated miRNAs in MPNSTs, we further analyzed the most markedly differentially expressed miRNAs (Table 2).
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In addition, extracellular matrix scaffolding molecules (collagens I and III) were markedly, but differentially, upregulated.
Treatment by the PKC activator phorbol 12-myristate 13-acetate (PMA) was found to markedly and differentially impair the up-regulation of estrogenic markers triggered by the estrogenic PAH benzanthracene (BZA) in cultured human mammary cells; BZA-mediated mRNA up-regulation of pS2 and amphiregulin was thus increased, whereas that of progesterone receptor and CXCL12 was repressed.
Conversely, the binding of UNG2 to RPA was markedly and differentially affected by the mutations.
Splice variants of Serum and Glucocorticoid-Inducible Kinase 1 (SGK1) which belongs to AGC group of protein kinase known to be regulated differentially following differentiation and shown to be markedly upregulated in tumor tissues [ 28].
However, it is unclear whether different diets modeled on popular weight loss programs which vary markedly in macronutrient composition differentially affect long term survival following breast cancer treatment [ 23- 35].
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