However, it should be noted that swimming speed did not change along acquisition sessions in either group of mice (MANOVA, "session": F(3 32) = 1.04, P = 0.39) and though significant in some sessions, the differences were not very marked (wild type: 12,15 cm.s−1 vs. Dyrk1A+/−: 13.14 cm.s−1, MANOVA, "session": F(1 16) = 2,182, P = 0.159).
Instead of a twin-clone analysis, we generated GFP marked wild type cells, since twin clone analysis was not reliable in testes GSCs (Fig. 4D,E).
We injected GFP marked wild-type (fl/fl), MSCKO and MSCKO expressing β-catenin K49R K345R subcutaneously into nude mice and scored for tumours.
In a scenario with 5 functional ISCs, a marked wild-type stem cell has a 1/5 (20%) chance to populate the whole crypt and replace all the other ISCs.
Follicle cell clones were generated as previously described (Duffy et al. 1998) using the e22c-Gal4 driver and enhanced GFP under the control of the ubiquitin promoter (ubi-eGFP) to mark wild-type cells.
While a control TRP1-marked wild-type histone H3 plasmid readily supported growth in orc5-1 and orc5-1 hat1Δ strains, a histone H3 multi-point mutant lacking acetylatable N-terminal lysines (K9, 14, 18, 23, 27R) conferred a severe loss of viability as indicated by the exclusively white colony color.
Furthermore the new Figure 3 figure supplement 1 confirms that the VE mutant of PPP1R15A, which is unable to engage PP1 stably, also interacts poorly with actin across a broad range of salt concentrations, in marked contrast to wild type PPP1R15A.
Arrowheads indicate pigment, which mark heterozygous or wild type control patches in the eye.
NF1 GISTs are marked by wild-type and multicentric tumors, are predominantly located in the small intestine, and are relatively indolent in terms of clinical and pathological features.
However, in LB broth shake cultures under experimental conditions used in this study, competitive fitness of the mutants was not statistically different from the competitive fitness of the wild type marked with an antibiotic resistance (data not shown).
Closed and open triangles mark, respectively, the wild type and mutant PPP1R15A lacking the C-terminal functional core.
