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# Through entire manuscript the Authors state, that there is "change in amino acids R- > K", but those residues are marked on sequence alignment to be in different places.
The new 3D alignment for a training course consists of measured individual elements from existing tracks combined with newly marked sequences of elements (generalized spline function).
The amino acids residues of PLldR and ELldR with predicted α-helices or β-sheets were then marked on the protein sequence alignment with magenta or yellow colors, according to the prediction results.
The location and phases of intron positions were determined within the above genes (Table 2) and marked on a multiple sequence alignment [ Additional File 6].
(B ) Putative transmembrane segments are highlighted with boxes, and mutated residues featured in this report are marked in color on the primary sequence alignment Y489 is shown in green and Q672 is shown in red.
Orthologous TAAR3, TAAR4 and TAAR5 full length nucleotide sequences of 14 mammalian species (Table S1 all species included are marked with *) were imported into Bioedit Sequence Alignment Editor 7.0.9 (http://www.mbio.ncsu.edu/BioEdit/bioedit.html) and aligned using ClustalW [14] followed by manual trimming as stated above.
The residues involved in the binding to the cofactor are marked with a triangle in the multiple sequence alignment.
Open image in new window Figure 2 Sequence alignment of HadA and HadB with secondary structural elements marked near the sequence.
The positions of the 8CM were retrieved with PAL2NAL (http://www.bork.embl.de/) by converting a multiple sequence alignment of 8CM sequences and the corresponding gene sequences into a codon alignment, and then marked in red [ 60].
The identification of loci marked by the two read stacks was achieved by sorting the mapped reads by base pair position in the sequence alignment map (SAM) file.
Second, if one side of the junction was marked as having only repeat sequence alignments during the phase when candidate junction sequences were constructed, then all of the ambiguous bases at the breakpoint are assigned to the side that had only unique alignments.
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